Immunohistochemistry

HistoFAXS unique features

- Automatic acquisition of an unlimited number of regions of interest on up to 200 slides
- Large overview images created from individual fields of view (FOV) may be exported at user-defined resolution
- Defines regions to be analyzed (or excluded from analysis) on the acquired regions of interest
- Semi-automated color separation to extract the relevant marker information out of immunohistochemical images
- Reliable automatic nuclear segmentation with minimum user interaction
- Forward Gating from the individual cell in the image to the dot in the scattergram as well as Backward Gating from the individual dot or dot group to the corresponding cells in the sample
- DotPlot operations – analysis of the image processing results via gating and additional DotPlots
- Histograms and overlays
- Statistics including percentage of positive cells, cells/mm2, and mean intensity

References

38.IL-4 induces cathepsin protease activity in tumor-associated macrophages to promote cancer growth and invasion.

Autoren:

Gocheva V, Wang HW, Gadea BB, Shree T, Hunter KE, Garfall AL, Berman T, Joyce JA.

Journal:

2010, Genes Dev, 2010 Jan 15. [Epub ahead of print]

37.Epidermal loss of JunB leads to a SLE phenotype due to hyper IL-6 signaling

Autoren:

Pflegerl P, Vesely P, Hantusch B, Schlederer M, Zenz R, Janig E, Steiner G, Meixner A, Petzelbauer P, Wolf P, Soleiman A, Egger G, Moriggl R, Kishimoto T, Wagner EF, Kenner L.

Journal:

2009, Proc Natl Acad Sci U S A. 106(48):20423-8. Epub 2009 Nov 16.

36.The potential role of c-Jun activation in patients with cutaneous lichen planus

Autoren:

Marceková Z, Flaig MJ, Kekuš M, Ruzicka T, Rupec RA.

Journal:

2009, Exp. Dermatol. Epub 2009 Sept 15

35.Myosin Light Chain Kinase Madiates Transcellular Intravasation of Breast Cancer Cells through the Underlying Endothelial Cells: A 3D FRET Study

Autoren:

Satya Khuon, Luke Liang, Robert Dettman, Peter H.S. Sporn, Robert B. Wysolmerski and Teng-Leong Chew

Journal:

2010, J Cell Sience, [Epub ahead of print]

34.COP1 Contributes to UVB-Induced Signaling in Human Keratinocytes.

Autoren:

Kinyó A, Kiss-László Z, Hambalkó S, Bebes A, Kiss M, Széll M, Bata-Csörgo Z, Nagy F, Kemény L

Journal:

2009, J Invest Dermatol. Epup 2009 Sept 10

33.Down-regulation of Suppressor of Cytokine Signaling-3 Causes Prostate Cancer Cell Death through Activation of the Extrinsic and Intrinsic Apoptosis Pathways.

Autoren:

Puhr M,Santer FR, Neuwirt H, Susani M, Nemeth JA, Hobisch A, Kenner L, and Culig Z

Journal:

2009, Cancer Res 69 (18), p. 7375-84. Epup 2009 Sept 8

32.Novel mechanism of IGF-binding protein-3 on prostate cancer cells: inhibition of proliferation, adhesion, and motility.

Autoren:

Petra Massoner, Daniela Colleselli, Andrea Matscheski1, Haymo Pircher, Stephan Geley, Pidder Jansen Dürr and Helmut Klocker

Journal:

2009, Endocrine-Related Cancer 16 1–15

31.Impaired autolysosome formation correlates with lamp-2 depletion: role of apoptosis, autophagy, and necrosis in pancreatitis.

Autoren:

Fortunato F, Bürgers H, Bergmann F, Rieger P, Büchler MW, Kroemer G, Werner J.

Journal:

2009, Gastroenterology 137(1):350-360.e5. Epub 2009 Apr 9

30.Coexpression of the melatonin receptor 1 and nestin in human breast cancer specimens.

Autoren:

Rögelsperger O, Ekmekcioglu C, Jäger W, Klimpfinger M, Königsberg R, Krenbek D, Sellner F, Thalhammer T.

Journal:

2009, J. Pineal Res 46:422-432

29.Suppressor of Cytokine Signaling (SOCS)-1 is Expressed in Human Prostate Cancer and Exerts Growth-Inhibitory Function through Down-Regulation of Cyclins and Cyclin-Dependent Kinases.

Autoren:

Neuwirt H, Puhr M, Santer FR, Susani M, Doppler W, Marcias G, Rauch V, Brugger M, Hobisch A, Kenner L, Culig Z.

Journal:

2009, Am J Pathol 174(5):1921-30. Epub 2009 Mar 26

28.Identification of micro-crystallin as an androgen-regulated gene in human prostate cancer.

Autoren:

Malinowska K, Cavarretta IT, Susani M, Wrulich OA, Uberall F, Kenner L, Culig Z.

Journal:

2009, Prostate 69(10):1109-1118. Epub 2009 Apr 7

27. Regulatory T cells are key cerebroprotective immunomodulators in acute experimental stroke.

Autoren:

Arthur Liesz, Elisabeth Suri-Payer, Claudia Veltkamp, Henrike Doerr, Clemens Sommer, Serge Rivest, Thomas Giese and Roland Veltkamp

Journal:

2009, Nature Medicine 15(2):192-9. Epub 2009 Jan 25

26. Monocytes/macrophages in kidney allograft intimal arteritis: no association with markers of humoral rejection or with inferior outcome.

Autoren:

Kozakowski N, Böhmig GA, Exner M, Soleiman A, Huttary N, Nagy-Bojarszky K, Ecker RC, Zeljko K, Regele H.

Journal:

2009, Nephrology Dialysis Transplantation 24(6):1979-86. Epub 2009 Feb 17

25. Insulin-Like Growth Factor Binding Protein-3 (IGFBP-3) in the Prostate and in Prostate Cancer: Local Production, Distribution and Secretion Pattern Indicate a Role in Stromal-Epithelial Interaction.

Autoren:

Massoner P, Haag P, Seifarth C, Jurgeit A, Rogatsch H, Doppler W, Bartsch G, Klocker H.

Journal:

2008, Prostate 68(11):1165-78.

24. Death receptor 5 mediated-apoptosis contributes to cholestatic liver disease.

Autoren:

Takeda K, Kojima Y, Ikejima K, Harada K, Yamashina S, Okumura K, Aoyama T, Frese S, Ikeda H, Haynes NM, Cretney E, Yagita H, Sueyoshi N, Sato N, Nakanuma Y, Smyth MJ, Okumura K.

Journal:

2008, Proc. Natl. Acad. Sci. USA: 105(31):10895-900

23. IL-17A enhances vitamin D3 induced expression of cathelicidin antimicrobial peptide in human keratinocytes.

Autoren:

Mark Peric, Sarah Koglin, Song-Min Kim, Shin Morizane, Robert Besch, Jörg C. Prinz, Thomas Ruzicka, Richard L. Gallo and Jürgen Schauber

Journal:

2008, Journal of Immunology, 181(12):8504-12.

22. The dark and the bright site of Stat3: proto-oncogene and tumor-suppressor.

Autoren:

A, Simma O, Hoelbl A, Kenner L, Beug H, Moriggl R, Sexl V.

Journal:

2009, Frontiers in Bioscience, 2009 Jan 1;14:2944-58

21. Oncogenic Kit controls neoplastic mast cell development through a Stat5/PI 3-kinase signaling cascade.

Autoren:

Harir N, Boudot C, Friedbichler K, Sonneck K, Kondo R, Kenner L, Kerenyi M, Gouilleux-Gruart V, Gondry J, Lassoued K, Valent P, Gouilleux F, Moriggl R.

Journal:

2008, Blood, Sep 15;112(6):2463-73; 20. Development of Pulmonary Fibrosis through a Pathway Involving the Transcription Factor Fra-2/AP-1.

Autoren:

Eferl R, Hasselblatt P, Rath M, Popper H, Zenz R, Komnenovic V, Idarraga MH, Kenner L and Wagner E.

Journal:

2008, Proc Natl Acad Sci USA, 105(30):10525-30 19. Lithium Promotes Adult Neural Progenitor Differentiation Via GSK3ß-Dependent Signaling Pathways.

Autoren:

Heinrich F. Bürgers, Dominik W. Schelshorn, Wolfgang Kuschinsky and Martin H. Maurer

Journal:

2008, The Open Enzyme Inhibition Journal, 1, 5-11 18. Acute anoxia stimulates proliferation in adult neural stem cells from the rat brain.

Autoren:

Bürgers HF, Schelshorn DW, Wagner W, Kuschinsky W, Maurer MH.

Journal:

2008, Experimental Brain Research 188:33-43

17. Protein expression differs between neural progenitor cells from the adult rat brain subventricular zone and olfactory bulb.

Autoren:

Martin H Maurer, Robert E Feldmann Jr, Heinrich F Bürgers and Wolfgang Kuschinsky

Journal:

2008, BMC Neuroscience Jan 16;9:7

16. Is Benign Prostatic Hyperplasia (BPH) an Immunoinflammatory Disease?

Autoren:

Gero Kramer, Dieter Mitteregger, Michael Marberger.

Journal:

2007, European Urology 51 (2007) 1202-1216 15. Glycogen Synthase Kinase 3ß (GSK3ß) Regulates Differentiation and Proliferation in Neural Stem Cells from the Rat Subventricular Zone.

Autoren:

Martin H. Maurer, Jens O. Brömme, Robert E. Feldmann, Jr., Anne Järve, Fatemeh Sabourl, Heinrich F. Bürgers, Dominik W. Schelshorn, Carola Krüger, Armin Schneider, and Wolfgang Kuschinsky

Journal:

2007, Journal of Proteome Research, 6, 1198-1208 14. 3D Parallel Coordinate Systems – A New Data Visualization Method in the Context of Microscopy-Based Multicolor Tissue Cytometry (MMTC).

Autoren:

Marc Streit, Rupert C. Ecker, Katja Österreicher, Georg E. Steiner, Horst Bischof, Christine Bangert, Tamara Kopp and Radu Rogojanu.

Journal:

2006, Cytometry A. Jul;69(7):601-11

13. An Improved Method for Discrimination of Cell Populations in Tissue Sections Using Microscopy-Based Multicolor Tissue Cytometry.

Autoren:

Rupert C. Ecker, Georg E. Steiner, Marc Streit, Katja Österreicher, Radu Rogojanu.

Journal:

2006, International Society for Analytical Cytology. Cytometry Part A 69A:119-123 (2006)

12. Leukocyte segmentation and classification in blood-smear images.

Autoren:

Herbert Ramoser, Vincent Laurain, Horst Bischof, Rupert Ecker.

Journal:

2005, Proc. Of the Int. Conf. Of the IEEE Engineering In Medicine and Biology Society (EMBS);

11. Fast Automatic Segmentation of Nuclei in Microscopy Images of Tissue Sections.

Autoren:

Vincent Laurain, Herbert Ramoser, Christoph Nowak, Rupert Ecker.

Journal:

2005, Proc. Of the Int. Conf. Of the IEEE Engineering In Medicine and Biology Society (EMBS);

10. Pimecrolismus leads to an apoptosis-induced depletion of T cells but not Langerhans cells in patients with atopic dermatitis.

Autoren:

Wolfram Hoetzenecker, MD, PhD, Rupert Ecker, PhD, Tamara Kopp, MD, Anton Stuetz, PhD, Georg Stingl, MD, and Adelheid Elbe-Bürger, PhD

Journal:

2005, Journal of Allergy and Clinical Immunology, 115(6):1276-83

9. Autocrine IL-10 partially prevents differentiation of neonatal dendritic epidermal leukocytes into Langerhans cells.

Autoren:

Souyet Chang-Rodriguez, Rupert C. Ecker, Georg Stingl, Adelhaid Elbe-Bürger

Journal:

2004, Journal of Leukocyte Biology, 76(3):657-66

8. Application of Spectral Imaging Microscopy in Cytomics and Fluorescence Resonance Energy Transfer (FRET) Analysis.

Autoren:

RC. Ecker, R de Martin, GE. Steiner, J A. Schmid.

Journal:

2004, Cytometry Part A 59A(2):182-90.

7. Microscopy-Based Multicolor Tissue Cytometry at the Single Cell Level.

Autoren:

Rupert C. Ecker and Georg E. Steiner

Journal:

2004, Cytometry, 59A(2):182-90, Abstract auf PubMed

6. Corticosteroids but not Pimecrolimus affect viability, maturation and immune function of murine epidermal Langerhans cells.

Autoren:

Wolfram Hoetzenecker, Josef G. Meingassner, Rupert C. Ecker, Georg Stingl, Anton Stuetz, Adelheid Elbe-Buerger

Journal:

2004, Journal of Investigative Dermatology, 122(3):673-84, Abstract auf PubMed

5. Inhibition of Restenosis by Tissue Factor Pathway Inhibitor: in vivo and in vitro evidence for suppressed Monocyte Chemoattraction and Gelatinase Activity.

Autoren:

05. W. Kopp, T Hölzenbein, S Steiner, R Marculescu, H Bergmeister, D Seidinger, I Mosberger, C Kaun, M Cejna, R Horvat, J Wojta, G Maurer, RC. Ecker, R de Martin, E Minar.

Journal:

2004, Blood 103(5):1653-61.

4. Overexpression of anti-CD75 reactive proteins on distal and collecting renal tubular epithelial cells in calcium-oxalate stone forming kidneys in Egypt.

Autoren:

Gero Kramer, Georg E. Steiner, Franz Reithmayer, Moshgan Prinz-Kashani, Markus Hohenfellner, Mohamed Gomha, Mohamed Ghoneim, Martin Newman and Michael Marberger

Journal:

2004, British Journal of Urology International, 93(6):822-826, Abstract auf PubMed

3. Cell-suface matrix proteins and sialic acids in cell-crystal adhesion; the effect of cristal binding on the viability of human CAKI-1 renal epithelial cells.

Autoren:

Gero Kramer, Georg E. Steiner, Moshgan Prinz-Kashani, Bernt Bursa, Michael Marberger

Journal:

2003, British Journal of Urology International, 91:554-559, Abstract auf PubMed

2. Histopathologische Quantifizierung immunologischer Prozesse.

Autoren:

Rupert C. Ecker, Rainer de Martin, and Georg E. Steiner

Journal:

2003, Biomedizinische Technik, 48 (Suppl. 1):546-547

1. Automated data acquisition by confocal laser scanning microscopy and image analysis of triple stained immunofluorescent leukocytes in tissue.

Autoren:

Georg Erich Steiner, Rupert Christian Ecker, Gero Kramer, Felix Stockenhuber, Michael Johann Marberger

Journal:

2000, Journal of Immunological Methods, 237(1-2):39-50

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